“基于叶绿体DNA序列的濒危植物宽叶羌活的遗传多样性研究”一文在Biochemical Systematics and Ecology 杂志上发表

Guoying Zhou（周国英）, Lucun Yang（杨路存）, Chunli Li（李春丽）, Wenhua Xu（徐文华）, Guichen Chen（陈桂琛）. Genetic diversity in endangered Notopterygium forbesii Boissieu based on intraspecies sequence variation of chloroplast DNA and implications for conservation, Biochemical Systematics and Ecology. 2010, 38 (5) : 911-916, IF=1.110

Abstract:  A thorough understanding of the levels and partitioning of genetic variation across populations and geographical regions of endangered species is a prerequisite to ensure effective conservation and/or restoration activities. Here, we examined chloroplast DNA (cpDNA) trnH-psbA intergenic spacer sequences variation within Notopterygium forbesii, an endangered and endemic perennial herb in China. Sequence data obtained from 141 individuals in 14 populations revealed twenty-two haplotypes. A high level of haplotype diversity (Hd = 0.81) and low level of nucleotide diversity (Pi = 0.0047) were detected. Low genetic differentiation among populations and also among regions was consistently indicated by both hierarchical analyses of molecular variance (AMOVA) and the structure of a neighbor-joining tree. Low level of population differentiation between populations or between regions in cpDNA sequences may be due to effects of the abundance of ancestral haplotype sharing and the high number of private haplotypes fixed for each population. Based on our results, we proposed some conservation strategies.

Keywords:  Microula sikkimensis　  Supercritical fluid extraction　  Orthogonal array design