“超临界CO₂萃取法提取锡金微孔草种子油”一文在Journal of the American Oil Chemists' Society 杂志上发表

Shi Lina, Ren Fei, Zhao Xudong, Du Yangong, Han Fa. Supercritical Carbon Dioxide Extraction of Microula sikkimensis Seed Oil, Journal of the American Oil Chemists' Society. 2010, 87:1221–1226, IF=1.803 

Abstract:

The seed oil of Microula sikkimensis had been intensively studied due to its pharmacological actions. In the present study, seed oil of Microula sikkimensis was extracted using supercritical fluid extraction (SFE). Determinations of the extracts composition were performed by gas chromatography (GC). An orthogonal array design (OAD), OA9 (34), was employed for optimization of the supercritical fluid extraction of the compound with regard to the various parameters. Four factors, namely pressure (21.0–27.0 MPa), the dynamic extraction time, temperature, and CO₂ flow rate of the supercritical fluid, were studied and optimized by a three-level OAD. The effects of the parameters on the yield of seed oil were studied using analysis of variance (ANOVA). The results revealed that the pressure had a significant effect on the yield of seed oil (p\0.05), while the other three factors, i.e., CO₂ flow rate, dynamic extraction time and temperature, were not identified as significant factors under the selected conditions based on ANOVA. The results show that the best values for the extraction condition of seed oil was pressure 24.0 MPa, extraction time 3 h, temperature 45 _C and a CO₂ flow rate 20 L/h in the 20-L vessel.

Keywords:

Microula sikkimensis

Supercritical fluid extraction

Orthogonal array design