“利用大孔树脂和制备液相色谱从毛诃子中分离没食子酸”一文在Journal of Chromatographic Science上发表

　　邹登朗，陈涛，陈晨，李洪梅，刘永玲，李玉林*. An Efficient Protocol for Preparation of Gallic Acid from Terminalia bellirica (Gaertn.) Roxb by Combination of Macroporous Resin and Preparative High-Performance Liquid Chromatography.Journal of Chromatographic Science, 2016, 1-5. IF=1.363

　　该研究首先利用静态和动态吸附和解吸附实验对D101、HPD-600、S-8、X-5、AB-8 和D3520六种树脂进行了研究。最终选择最优树脂AB-8进行没食子酸的富集，回收率达到85%。然后利用制备液相色谱进行分离，并对流速和上样量进行了优化，最终分离得到没食子酸，纯度达到98%以上。

　　Abstract: In this article, macroporous resin column chromatography and preparative high-performance liquid chromatography were applied for preparation of gallic acid from Terminalia bellirica (Gaertn.) Roxb. In the first step, six kinds of resins were investigated by adsorption and desorption tests and AB-8 macroporous resin was selected for the enrichment of gallic acid. As a result, 20 g of gallic acid at a purity of 71% could be separated from 100 g of crude extract in which the content of gallic acid was 16.7% and the recovery of gallic acid reached 85.0%. In the second step, preparative highperformance liquid chromatography was selected to purify gallic acid. As a result, 640 mg of gallic acid at a purity of 99.1% was obtained from 1 g of sample in 35 min. The results demonstrated that macroporous resin coupled with preparative high-performance liquid chromatography was suitable for preparation of gallic acid from Terminalia bellirica (Gaertn.) Roxb.