新闻动态

“CD光盘分析测定铅离子” 一文在Analytical Chemistry杂志上发表

2011-06-13  科技处

Honglun Wang(王洪伦),Lily M. L. Ou, Yourui Suo(索有瑞),Hua-Zhong Yu(于化忠).Computer-Readable DNAzyme Assay on Disc for ppb-Level Lead Detection, Analytcial Chemistry. 2011, 83, 1557-1563,IF=5.874(全文链接见附件)

Abstract:A method for the on-site detection of lead at the parts-per-billion (ppb)-level has been developed; it uses a conventional compact disc (CD) as the platform for preparing DNAzyme assays and an unmodified optical drive of ordinary desktop/laptop computers as the readout device. In particular, by immobilizing PbP2+P-specific DNAzyme sensing constructs on the “transparent side” of a conventional CD-R via mild surface reactions, the PbP2+P concentration can be determined by a free diagnostic program that checks the error distribution on the CD (i.e., it extracts the number of errors in a pre-recorded audio file). The reading errors increases monotonically over a wide range of PbP2+P concentrations (from 10 nM to 1 mM) and the selectivity is confirmed by testing several other divalent cations (ZnP2+P, BaP2+P, MgP2+P, CaP2+P, CuP2+P, HgP2+P).

Computer-Readable DNAzyme Assay on Disc for ppb-Level Lead Detection.pdf